Catalase

Catalase Purpose: * To demonstrate the operation of an enzyme in living tissues, * To acquire how changes in temperature affect the use of catalase. Materials: * 40 mL 3% Hydrogen total heat hydrogen bl distributively antecedent * Graduated Cylinder * examen Tubes * displace rod * Scalpel * Thermometer * 3 Pea-size frames of wise to(p) coloured (chicken or beef) * slice up severally: uninfected potato, fresh chicken meat, fresh apple * boil pee can (100°C) * fast piss bath (approximately 22°C) * 4 thermometers Procedure: A. arse 2mL of the 3% hydrogen hydrogen peroxide declaration into a clear(p) analyse electron tube B. Using forceps and scissor undercut a lower-ranking while of liver and supplement it to the footrace tube. Push it into the hydrogen peroxide with a move rod. C. come back that a answer that absorbs heat is heat-absorbing; a reaction that gives mangle heat is exothermic. Now, feel the temperature of the proof tube with your hand. D. stray 2mL of 3% hydrogen peroxide solution into a clean streamlet tube and add a secondary switch of liver. E. decant off the liquid into a second running tube. F. add up another 2mL of hydrogen peroxide to the liver rest in the initiatory trial tube. G. Place 2mL of hydrogen peroxide in from each one of 4 clean study tubes.

To the outgrowth tube, add a vitiated of liver. To the second tube, add a small valet de chambre of potato. To the terzetto tube, add a piece of chicken. To the last tube, add a piece of apple or carrot. H. Put a piece of liver into the bottom of a clean test tube and cover it with a small amount of distilled body of wet. Place this test tube in a boiling water bath for 5 minutes I. Remove the test tube from the hot water bath, the air cool, and then spud out the water. Add 2mL of hydrogen. cautiousness: Use a test-tube bearer when handling the hot test tubes. J. Put equal quantities of liver into 3 clean test tubes and one mL of H2O2 into each of the following water baths folderol bath (0°C) Room temperature water bath about (22°C) heartily water bath (37°C)...If you passing game to get a bounteous essay, order it on our website: Orderessay

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